(1) Kobayashi J, Hasegawa T, Sugeno N, Yoshida S, Akiyama T,
Fujimori K, Hatakeyama H, Miki Y,
Tomiyama A, Kawata Y, Fukuda M, Kawahata I,
Yamakuni T, Ezura M, Kikuchi A, Baba T, Takeda A,
Kanzaki M, Wakabayashi K, Okano H, Aoki M,
Extracellular α-synuclein enters dopaminergic cells by
modulating flotillin-1–assisted dopamine transporter
endocytosis
The FASEB JOURNAL Published:18 Jun 2019
https://doi.org/10.1096/fj.201802051R
(2) Kawano S, Ito k, Yahata K, Kira K, Abe T, Akagi, T, Asano M,
Iso K, Sato Y, Matsuura F, Ohashi I,
A landmark in drug discovery based on complex natural
product synthesis
Scientific Reports, Published: 17 June 2019
https://doi.org/10.1038/s41598-019-45001-9
(3) Janet Tan S, Nakahara K, Sou K, Takeoka S,
An Assay to Evaluate the Function of Liposomal Platelet
Substitutes Delivered to Platelet Aggregates
Front. Bioeng. Biotechnol, Published: 12 April 2019,
https://doi.org/10.3389/fbioe.2019.00077
(4) Mashima K, Takahashi S, Minami K, Izawa Y, Abe T,
Tsukada N, Hishiki T, Suematsu M, Kajimura M,
Suzuki N,
Neuroprotective Role of Astroglia in Parkinson Disease by
Reducing Oxidative Stress Through Dopamine-Induced
Activation of Pentose-Phosphate Pathway
SAGE journals, Published May 16, 2018,
https://doi.org/10.1177/1759091418775562
(5) Aoki M, Das D, Hayashi H, Aoki-Ogata H, Takamatsu Y,
Arun K. Ghosh, Mitsuya H
Mechanism of Darunavir (DRV)’s High Genetic Barrier to
HIV-1 Resistance: A Key V32I Substitution in Protease
Rarely Occurs, but Once It Occurs, It Predisposes HIV-1 To
Develop DRV Resistance
ASM Journals, Published 6 March 2018,
DOI: 10.1128/mBio.02425-17
(6) Usui K, Mie M, Andou T, Mihara H, Kobatake E
Fluorescent and luminescent fusion proteins for analyses of
amyloid beta peptide aggregation
Journal of Peptide Science Volume 23, Issue 7-8 July-August
2017 Pages 659–665
(7) Mon H, Lee JM, Sato M, Kusakabe T
Identification and functional analysis of outer kinetochore
genes in the holocentric insect Bombyx mori
Insect Biochemistry and Molecular Biology Volume 86, July 2017, Pages 1-8
https://doi.org/10.1016/j.ibmb.2017.04.005
(8) Aoki M, Hayashi H, Rao KV, Das D, Higashi-Kuwata N,
Bulut H, Aoki-Ogata H, Takamatsu Y, Yedidi RS, Davis DA,
Hattori S, Nishida N, Hasegawa K, Takamune N, Nyalapatla
PR, Osswald HL, Jono H, Saito H, Yarchoan R, Misumi S,
Ghosh AK, Mitsuya H
A novel central nervous system-penetrating protease
inhibitor overcomes human immunodeficiency virus 1
resistance with unprecedented aM to pM potency
eLife. 2017; 6: e28020. doi: 10.7554/eLife.28020
(9) Nakamura T, Campbell JR, Moore AR, Otsu S, Aikawa H,
Tamamura H, Mitsuya H
Development and validation of a cell-based assay system to
assess human immunodeficiency virus type 1 integrase
multimerization
Journal of Virological Methods Volume 236, October 2016,
Pages 196-206
https://doi.org/10.1016/j.jviromet.2016.07.023
(10) Aoki M, Hayashi H, Yedidi RS, Martyr CD, Takamatsu Y,
Aoki-Ogata H, Nakamura T, Nakata H, Das D, Yamagata Y,
Ghosh AK, Mitsuya H
C-5-Modified Tetrahydropyrano-Tetrahydofuran-Derived
Protease Inhibitors (PIs) Exert Potent Inhibition of the
Replication of HIV-1 Variants Highly Resistant to Various
PIs, including Darunavir
Journal of Virology March 2016 vol. 90 no. 5 2180-2194
(11) Iizumi T, Takahashi S, Mashima K, Minami K, Izawa Y, Abe T,
Hishiki T, Suematsu M, Kajimura M, Suzuki N
A possible role of microglia-derived nitric oxide by
lipopolysaccharide in activation of astroglial pentose-phosphate pathway via the Keap1/Nrf2 system
Journal of Neuroinflammation 201613:99
https://doi.org/10.1186/s12974-016-0564-0
(12) Ando J, Asanuma M, Dodo K, Yamakoshi H, Kawata S,
Fujita K, Sodeoka M
Alkyne-Tag SERS Screening and Identification of Small-Molecule-Binding Sites in Protein
Journal of the American Chemical Society 2016,
138 (42), pp 13901–13910 DOI: 10.1021/jacs.6b06003
(13) Naito H, Goshima G
NACK Kinesin Is Required for Metaphase Chromosome
Alignment and Cytokinesis in the Moss Physcomitrella
Patens
Cell Structure and Function Vol. 40 (2015) No. 1 p. 31-41
(14) Takahashi H, Yanagisawa T, Kim KS, Yokoyama S, Ohnishi M
Multiple Functions of Glutamate Uptake via Meningococcal
GltT-GltM l-Glutamate ABC Transporter in Neisseria
meningitidis Internalization into Human Brain
Microvascular Endothelial Cells
Infect. Immun. September 2015 vol. 83 no. 9, 3555-3567
(15) Nakagawa T, Ohta K, Kubozono K, Ishida Y, Naruse T,
Takechi M, Kamata N
Zoledronate inhibits receptor activator of nuclear factor
kappa-B ligand-induced osteoclast differentiation via
suppression of expression of nuclear factor of activated
T-cell c1 and carbonic anhydrase 2
Archives of Oral Biology Volume 60, Issue 4, April 2015,
Pages 557–565
Keywords: Bisphosphonate, Zoledronate, DNA microarray,
RANKL, NFATc1, CAII
(16) Edzuka T, Yamada L, Kanamaru K, Sawada H, Goshima G
Identification of the Augmin Complex in the Filamentous
Fungus Aspergillus nidulans
PLoS ONE 9(7): e101471. doi:10.1371/journal.pone.0101471(2014)
(17) Chowdhury MM, Danoy M, Rahman F, Shinohara M,
Kaneda S, Shiba K, Fujita N, Fujii T, Sakai Y
Adhesion of Pancreatic Cancer Cells in a Liver-Microvasculature Mimicking Coculture Correlates with
Their Propensity to Form Liver-Specific Metastasis In
Vivo
BioMed Research International Volume 2014 (2014), Article
ID 241571, 13 pages
(18) Kobayashi D, Hirayama M, Komohara Y, Mizuguchi
S, Morifuji M, Ihn H, Takeya M, Kuramochi A, Araki N
Translationally Controlled Tumor Protein Is a Novel
Biological Target for Neurofibromatosis Type 1-associated
Tumors
The Journal of Biological Chemistry, 289, 26314-26326.
September 19, 2014
(19) Toda S, Segawa K, Nagata S
MerTK-mediated engulfment of pyrenocytes by central
macrophages in erythroblastic islands
Blood: 123 (25), June 19, 2014
(20) Stoehr A, Neuber C, Baldauf C, Vollert I, Friedrich FW,
Flenner F, Carrier L, Eder A, Schaaf S, Hirt MN,
Aksehirlioglu B, Tong CW, Moretti A, Eschenhagen T,
Hansen A
Automated analysis of contractile force and Ca2+
transients in engineered heart tissue
American Journal of Physiology - Heart and Circulatory
Physiology Published 1 May 2014 Vol. 306 no. 9,
H1353-H1363 DOI: 10.1152/ajpheart.00705.2013
(21) Tran TT, Tobiume K, Hirono C, Fujimoto S, Mizuta K,
Kubozono K, Inoue H, Itakura M, Sugita M, Kamata N
TMEM16E (GDD1) Exhibits Protein Instability and
Distinct Characteristics in Chloride Channel/Pore
Forming Ability
Journal of Cellular Physiology Volume 229, Issue 2, pages
181–190, February 2014
(22) Echizen K, Nakada M, Hayashi T, Sabit H, Furuta T, Nakai M,
Koyama-Nasu R, Nishimura Y, Taniue K,
Morishita Y, Hirano S, Terai K, Todo T, Ino Y, Mukasa A,
Takayanagi S, Ohtani R, Saito N, Akiyama T
PCDH10 is required for the tumorigenicity of glioblastoma
cells
Biochemical and Biophysical Research Communications
Volume 444, Issue 1, 31 January 2014, Pages 13–18
(23) Sato Y, Mukai M, Ueda J, Muraki M, Stasevich TJ,
Horikoshi N, Kujirai T, Kita H, Kimura T, Hira S, Okada Y,
Hayashi-Takanaka Y, Obuse C, Kurumizaka H, Kawahara A,
Yamagata K, Nozaki N, Kimura H
Genetically encoded system to track histone modification
in vivo
SCIENTIFIC REPORTS | 3 : 2436 | DOI: 10.1038/srep02436(2013)
(24) Rizqiawan A, Tobiume K, Okui G, Yamamoto K, Shigeishi H,
Ono S, Shimasue H, Takechi M, Higashikawa K,
Kamata N
Autocrine galectin-1 promotes collective cell migration of
squamous cell carcinoma cells through up-regulation of
distinct integrins
Biochemical and Biophysical Research Communications
Volume 441, Issue 4, 29 November 2013, Pages 904–910
Keywords: EMT, Snail, Galectin-1, Collective cell migration
(25) Okui G, Tobiume K, Rizqiawan A, Yamamoto K, Shigeishi H, Ono S, Higashikawa K, Kamata N
AKT primes snail-induced EMT concomitantly with the
collective migration of squamous cell carcinoma cells
Journal of Cellular Biochemistry Volume 114, Issue 9
September 2013 Pages 2039–2049
(26) Yamauchi N, Gosho T, Asatuma S, Toyooka K, Fujiwara T,
Matsuoka K
Polarized localization and borate-dependent degradation
of the Arabidopsis borate transporter BOR1 in tobacco
BY-2 cells
F1000Res. 2013; 2: 185. Published online 2013 Sep 13. doi:
10.12688/f1000research.2-185.v1
(27) Che XF, Moriya S, Zheng CL, Abe A, Tomoda A,
Miyazawa K
2-Aminophenoxazine-3-one-induced apoptosis via
generation of reactive oxygen species followed by c-jun
N-terminal kinase activation in the human glioblastoma
cell line LN229
International Journal of Oncology Volume 43 Issue 5 Pages:
1456-1466 DOI: 10.3892/ijo.2013.2088
(28) Stöhr A, Friedrich FW, Flenner F, Geertz B, Eder A,
Schaaf S, Hirt MN, Uebeler J, Schlossarek S, Carrier L,
Hansen A, Eschenhagen T
Contractile abnormalities and altered drug response in
engineered heart tissue from Mybpc3-targeted knock-in
mice
Journal of Molecular and Cellular Cardiology Volume 63,
October 2013, Pages 189–198
(29) Tanaka T, Kuroda K, Ikeda M, Wakita T, Kato N,
Makishima M
Hepatitis C virus NS4B targets lipid droplets through
hydrophobic residues in the amphipathic helices
April 2013 The Journal of Lipid Research, 54, 881-892.
(30) Shimada H, Nambu-Niibori A, Wilson-Morifuji M,
Mizuguchi S, Araki N, Sumiyoshi H, Sato M, Mezaki Y,
Senoo H, Ishikawa K, Hatano Y, Okamoto O, Fujiwara S
Epiplakin modifies the motility of the HeLa cells and
accumulates at the outer surfaces of 3-D cell clusters
The Journal of Dermatology Volume 40, Issue 4,
pages 249–258, April 2013
Keywords: cell migration, epiplakin, HeLa cells,
overexpression, 3-D culture, zonula occludens-1
(31) Nitta H, Wada Y, Kawano Y, Murakami Y, Irie A, Taniguchi K,
Kikuchi K, Yamada G, Suzuki K, Honda J,
Wilson-Morifuji M, Araki N, Eto M, Baba H, Imamura T
Enhancement of Human Cancer Cell Motility and
Invasiveness by Anaphylatoxin C5a via Aberrantly
Expressed C5a Receptor (CD88)
Clin Cancer Res April 15, 2013 19; 2004
(32) Khaenam P, Niibori A, Okada S, Jearanaikoon P, Araki N,
Limpaiboon T
Contribution of RIZ1 to Regulation of Proliferation and
Migration of a Liver Fluke-Related Cholangiocarcinoma
Cell
Asian Pacific Organization for Cancer Prevetion Volume 13,
Issue 8, 2012, pp.4007-4011
(33) Aoki M, Danish ML, Aoki-Ogata H, Amano M, Ide K, Das D,
Koh Y, Mitsuya H
Loss of the Protease Dimerization Inhibition Activity of
Tipranavir (TPV) and Its Association with the Acquisition
of Resistance to TPV by HIV-1
J. Virol. December 2012 vol. 86 no. 24 13384-13396
(34) Takahashi S, Izawa Y, Suzuki N
Astroglial Pentose Phosphate Pathway Rates in Response
to High-Glucose Environments
ASN Neuro February 2012 vol. 4 no. 2 AN20120002
(35) Nakao Y, Kimura H, Sakai Y, Fujii T
Bile canaliculi formation by aligning rat primary
hepatocytes in a microfluidic device
Biomicrofluidics 5, 022212 (2011);
http://dx.doi.org/10.1063/1.3580753
(36) Koh Y, Aoki M, Danish ML, Aoki-Ogata H, Amano M, Das D,
Shafer RW, Ghosh AK, Mitsuya H
Loss of Protease Dimerization Inhibition Activity of
Darunavir Is Associated with the Acquisition of Resistance
to Darunavir by HIV-1
J. Virol. October 2011 vol. 85 no. 19 10079-10089
(37) Kasahara E, Sekiyama A, Hori M, Hara K, Takahashi N,
Konishi M, Sato EF, Matsumoto S, Okamura H, Inoue M
Mitochondrial density contributes to the immune response
of macrophages to lipopolysaccharide via the MAPK
pathway
FEBS Letters Volume 585, Issue 14, 21 July 2011, Pages
2263–2268
Keywords: Mitochondria, Innate immunity, Inflammatory
cytokine, Macrophage, Reactive oxygen species, TLR4
(38) Ohto-Fujita E, Konno T, Shimizu M, Ishihara K, Sugitate T, Miyake J, Yoshimura K, Taniwaki K, Sakurai T
Hydrolyzed eggshell membrane immobilized on
phosphorylcholine polymer supplies extracellular matri
environment for human dermal fibroblasts
Cell and Tissue Research July 2011, Volume 345, Issue 1, pp 177-190
(39) Ide K, Aoki M, Amano M, Koh Y, Yedidi RS, Das D,
Leschenko S, Chapsal B, Ghosh AK, Mitsuya H
Novel HIV-1 protease inhibitors (PIs) containing a bicyclic
P2 functional moiety, tetrahydropyrano-tetrahydrofuran,
that are potent against multi-PI-resistant HIV-1 variants
Antimicrob. Agents Chemother. April 2011 vol. 55 no. 4
1717-1727
(40) Obara M, Sakuma T, Fujikawa K
The third type III module of human fibronectin mediates
cell adhesion and migration
J Biochem (2010) 147 (3): 327-335. doi: 10.1093/jb/mvp168
(41) Tojo Y, Koh Y, Amano M, Aoki M, Das D, Kulkarni S,
Anderson DD, Ghosh AK, Mitsuya H
Novel Protease Inhibitors (PIs) Containing
Macrocyclic Components and 3(R),3a(S),6a(R)-bis-Tetrahydrofuranylurethane That Are Potent against
Multi-PI-Resistant HIV-1 Variants In Vitro
Antimicrob. Agents Chemother. August 2010 vol. 54 no. 8
3460-3470
(42) Aung W, Hasegawa S, Koshikawa-Yano M, Tsuji AB,
Sogawa C, Sudo H, Sugyo A, Koizumi M, Furukawa T, Saga T
Noninvasive assessment of regulable transferred-p53 gene
expression and evaluation of therapeutic response with
FDG–PET in tumor model
Gene Therapy (2010) 17, 1142–1151; doi:10.1038/gt.2010.70
Keywords: tumor-suppressor gene p53, optical imaging,
FDG–PET imaging
(43) Harada S, Sekiguchi N, Shimizu N
Amplification of a plasmid bearing a mammalian
replication initiation region in chromosomal and
extrachromosomal contexts
Nucleic Acids Research, 2010, 1–12 doi:10.1093/nar/gkq882
(44) Saitoh Y, Hongwei W, Ueno H, Mizuta M , Nakazato M
Candesartan attenuates fatty acid-induced oxidative stress
and NAD(P)H oxidase activity in pancreatic β-cells
Diabetes Research and Clinical Practice Volume 90, Issue 1,
October 2010, Pages 54–59
Keywords: Angiotensin II receptor blockers, Pancreatic
β-cells, Oxidative stress, PKC, NAD(P)H oxidase activities
(45) Hayashi-Takanaka Y, Yamagata K, Nozaki N, Kimura H
Visualizing histone modifications in living cells:
spatiotemporal dynamics of H3 phosphorylation during
interphase
JCB vol. 187 no. 6 781-790/December 7, 2009
(46) Saitoh Y, Hongwei W, Ueno H, Mizuta M, Nakazato M
Telmisartan attenuates fatty-acid-induced oxidative stress
and NAD(P)H oxidase activity in pancreatic β-cells
Diabetes & Metabolism Volume 35, Issue 5, November 2009,
Pages 392–397
(47) Kobayashi D, Kumagai J, Morikawa T, Wilson-Morifuji M,
Wilson A, Irie A, Araki N
An Integrated Approach of Differential Mass Spectrometry
and Gene Ontology Analysis Identified Novel Proteins
Regulating Neuronal Differentiation and Survival
Molecular & Cellular Proteomics, 8, 2350-2367, October 1, 2009
(48) Morin P, Flors C, Olson MF
Constitutively active RhoA inhibits proliferation by
retarding G1 to S phase cell cycle progression and
impairing cytokinesis
European Journal of Cell Biology Volume 88, Issue 9,
September 2009, Pages 495–507
(49) Tokumoto Y, Horimoto K, Miyake J
TRAIL inhibited the cyclic AMP responsible element
mediated gene expression
Biochemical and Biophysical Research Communications
Volume 381, Issue 4, 17 April 2009, Pages 533–536
(50) Yamada S, Hakamada K, Munekata T, Takano K, Fujita S,
Miyake M, Miyake J
A system for analyzing the event timing profile of single
cells by using a model of neurite maturation in PC12D
cells
Biosensors and Bioelectronics Volume 24, Issue 5, 1 January
2009, Pages 1493–1497
Keywords; Nerve growth factor, Neuritogenesis, Neurite
outgrowth, Single-cell analysis, Staurosporine
(51) Robertson AM, Hagan IM
Stress-regulated kinase pathways in the recovery of tip
growth and microtubule dynamics following osmotic stress
in S. pombe
December 15, 2008 J Cell Sci 121, 4055-4068.
(52) Saito Y, Furukawa T, Arano Y, Fujibayashi Y, Saga T
Comparison of semiquantitative fluorescence imaging
and PET tracer uptake in mesothelioma models as a
monitoring system for growth and therapeutic effects
Nuclear Medicine and Biology Volume 35, Issue 8, November
2008, Pages 851–860
Keywords: Mesothelioma, Mouse tumor model, Fluorescence
imaging, PET, FLT
(53) Ogura Y, Abe H, Katsura K, Kurokawa K, Asadulghani MD,
Iguchi A, Ooka T, Nakayama K, Yamashita A,
Hattori M, Tobe T, Hayashi T
Systematic Identification and Sequence Analysis of the
Genomic Islands of the Enteropathogenic Escherichia coli
Strain B171-8 by the Combined Use of Whole-Genome
PCR Scanning and Fosmid Mapping
J. Bacteriol. November 2008 vol. 190 no. 21 6948-6960
(54) Ikeda M, Ueno S, Matsumoto S, Shimizu Y, Komatsu H,
Kusumoto K, Hamachi I
Three-Dimensional Encapsulation of Live Cells by Using
a Hybrid Matrix of Nanoparticles in a Supramolecular
Hydrogel
Chemistry - A European Journal Volume 14, Issue 34, pages
10808–10815, November 26, 2008
(55) Saitoh Y, Chun-ping C, Noma K, Ueno H, Mizuta M,
Nakazato M
Pioglitazone attenuates fatty acid–induced oxidative stress
and apoptosis in pancreatic β-cells
Diabetes, Obesity and Metabolism Volume 10, Issue 7, pages
564–573, July 2008
(56) Patrakitkomjorn S, Kobayashi D, Morikawa T, Wilson MM,
Tsubota N, Irie A, Ozawa T, Aoki M, Arimura N, Kaibuchi K, Saya H, Araki N
Neurofibromatosis type 1 (NF1) tumor suppressor,
neurofibromin, regulates the neuronal differentiation of
PC12 cells via its associating protein, CRMP-2
J Biol Chem. 2008 Apr 4;283(14):9399-413.
Key word: collagen coated, rat PC12 cells, time-lapse video
analysis
(57) Wang X, Uto T, Akagi T, Akashi M, Baba M
Poly(γ-glutamic acid) nanoparticles as an efficient antigen
delivery and adjuvant system: Potential for an AIDS
vaccine
Journal of Medical Virology Volume 80, Issue 1, pages 11–19,
January 2008
(58) Koh Y, Matsumi S, Das D, Amano M, Davis DA, Li J,
Leschenko S, Baldridge A, Shioda T, Yarchoan R, Ghosh AK,
Mitsuya H
Potent Inhibition of HIV-1 Replication by Novel
Non-peptidyl Small Molecule Inhibitors of Protease
Dimerization
J Biol Chem. 2007 Sep 28;282(39):28709-20.
Key word: COS7 cells, FRET
(59) Takahashi M, Motohide Murate M, Mitsunori Fukuda M,
Sato SB, Ohta A, Kobayashi T
Cholesterol Controls Lipid Endocytosis through Rab11
Mol. Biol. Cell July 1, 2007 vol. 18 no. 7 2667-2677
(60) Oike H, Nagai T, Furuyama A, Okada S, Aihara Y, Ishimaru Y,
Marui T, Matsumoto I, Misaka T, Abe K
Characterization of Ligands for Fish Taste Receptors
The Journal of Neuroscience, May 23, 2007 • 27(21):
5584–5592
(61) Sultana N, Nag K, Kato A, Hirose S
Pillar cell and erythrocyte localization of fugu ETA
receptor and its implication
Biochemical and Biophysical Research Communications
Volume 355, Issue 1, 30 March 2007, Pages 149–155
(62) Uto T, Wang X, Sato K, Haraguchi M, Akagi T, Akashi M,
Baba M
Targeting of antigen to dendritic cells with poly(gamma-glutamic acid) nanoparticles induces antigen-specific
humoral and cellular immunity
J Immunol. 2007 Mar 1;178(5):2979-86.
Key word: mouse immature dendritic cells, confocal laser
scanning, Microscopy
(63) Nakae K, Hosokawa N, Sawa R, Kubota Y, Masuda T, Ohba S, Igarashi M,Nakagawa N, Nishimura Y, Akamatsu Y
A New Teleocidin Analog from Streptomyces sp. MM216-
87F4 Induces Substance P Release from Rat Dorsal Root
Ganglion Neurons
J. Antibiot. 59(1): 11–17, 2006
Key word: CHO Cells, laser-scanning confocal microscopy(GFP)
(64) Asoh S, Mori T, Nagai S, Yamagata K, Nishimaki K, Miyato Y, Shidara Y, Ohta S
Zonal necrosis prevented by transduction of the artificial
anti-death FNK protein
Cell Death and Differentiation (2005) 12, 384–394.
doi:10.1038/sj.cdd.4401569
Keywords: necrosis, apoptosis, protein transduction domain,
carbon tetrachloride, HepG2, liver, Bcl-xL, protein
therapeutics
(65) Wang X, Uto T, Sato K, Ide K, Akagi T, Okamoto M, Kaneko T, Akashi M, Baba M
Potent activation of antigen-specific T cells by antigen-loaded nanospheres
Immunology Letters Volume 98, Issue 1, 15 April 2005, Pages
123–130
Keywords: Polystyrene, Nanotechnology, Dendritic cell,
Maturation, Vaccine, HIV-1
(66) Chang FH, Lee CH, Chen MT, Kuo CC, Chiang YL,
Hang CY, Roffler S
Surfection: a new platform for transfected cell arrays
Nucl. Acids Res. (2004) 32 (3): e33. doi: 10.1093/nar/gnh029
